monoclonal mouse anti fetuin a  (Santa Cruz Biotechnology)

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Overexpression of fetuin-A (Fet-A) in MDA-MB-468 TNBC cell line. MDA-MB-468 cells were transfected with either empty vector (EV) or flag-tagged fetuin-A expression vector (FA). Fetuin-A expression level was determined by QT-RTPCR (panel A), Western blot (panel B) and IHC (panel C) as described in .

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Enhanced invasion capacity in fetuin-A overexpressing MDA—MB-468 cells. The MDA-MB-468-FA invaded through a bed of Matrigel more readily compared to MDA-MB-468-EV when complete medium (CM) was in the lower compartment of the trans-well (*P<0.05); N=6). Both sub-clones did not invade when SFM was in the lower compartments (controls) (**P<0.001; N=6)

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Clone Assay

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Toll like receptor 4 (TLR4) expression in MDA-MB-468 sub-clones. Surface TLR4 expression levels were determined by flow cytometry in MDA-MB-468-EV (upper panels) and MDA-MB-468-FA (lower panels) in the absence and presence of added fetuin-A (FA)

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Expressing, Clone Assay, Flow Cytometry

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Fetuin-A mediates 2-D and 3-D growth of MDA-MB-468 via TLR4 signaling. The cells were seeded (2 × 10 4 cells/well) in attachment (2-D) ( panel A ) or ultra-low attachment (3-D) ( panel B ) 96-well plates in SFM; SFM + CLI-095 (CLI); Fetuin-A (FA); FA + CLI; complete medium (CM); and in CM + CLI. After 6 days of growth, Alamar blue was added to each well, incubated for 2 h and fluorescence measured as described. *P< 0.05; **P< 0.001; ***P<0.0001; N= 4

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Incubation, Fluorescence

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: In A , control chambers had only cells in SFM, while experimental chambers had CLI-095 (28 µM) in SFM (upper chambers) of trans-well assay. The bottom chambers had complete medium (CM). In B , controls had CM only in the bottom chambers while experimental chambers had CM depleted of fetuin-A (CM + HA). Number of invading cells was determined as described in .

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Annexins A2, A6 and Fetuin-A Affect the Process of Mineralization in Vesicles Derived from Human Osteoblastic hFOB 1.19 and Osteosarcoma Saos-2 Cells

doi: 10.3390/ijms22083993

Figure Lengend Snippet: Co-localization of AnxA6, AnxA2, or fetuin-A (FetuA) with TNAP in hFOB 1.19 ( A , C , E ) and Saos-2 ( B , D , F ) cells in resting conditions (R) or after seven-day stimulation with AA and β-GP (S). The cells were incubated with appropriate antibodies: mouse monoclonal anti-AnxA6 ( A , B ), mouse monoclonal anti-AnxA2 ( C , D ), or mouse monoclonal anti-FetuA ( E , F ), all followed by goat anti-mouse IgG-FITC (green); rabbit polyclonal anti-TNAP followed by goat anti-rabbit IgG-TRITC (red) and DAPI for nuclei (blue) and observed under an Axio Observer.Z1 FM (Carl Zeiss, Poznan, Poland) with Phase contrast and appropriate fluorescent filters, magnification 630 x. Arrowheads indicate protein accumulation in vesicular and/or cluster structures. The yellow color and arrows on the merge images indicate AnxA6 ( A , B ), AnxA2 ( C , D ), or FetuA ( E , F ) co-localization with TNAP. Results of a typical experiment are presented.

Article Snippet: Then, cells were incubated with mouse monoclonal anti-annexin A6 (AnxA6; 1:100, v/v ; BD Transduction Laboratories, Warsaw, Poland), mouse monoclonal anti-annexin A2 (AnxA2; 1:100, v/v ; BD Transduction Laboratories, Warsaw, Poland), mouse monoclonal anti-fetuin-A (FetuA; 1:100, v/v ; Abcam, Cambridge, UK), or rabbit polyclonal anti-TNAP (TNAP; 1:100, v/v ; Abcam, Cambridge, UK) primary antibodies diluted in TBS containing 0.5% FBS ( v/v ) and 0.05% Tween-20 ( v/v ).

Techniques: Incubation